Protein_Domain

Part:BBa_K4161017:Design

Designed by: Chang Shen   Group: iGEM22_DKU   (2022-08-18)


Neae: the intimin fragment for surface display


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 105
    Illegal PstI site found at 401
    Illegal PstI site found at 649
    Illegal PstI site found at 1096
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 105
    Illegal PstI site found at 401
    Illegal PstI site found at 649
    Illegal PstI site found at 1096
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 105
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 105
    Illegal PstI site found at 401
    Illegal PstI site found at 649
    Illegal PstI site found at 1096
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 105
    Illegal PstI site found at 401
    Illegal PstI site found at 649
    Illegal PstI site found at 1096
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1244


Design Notes

A FE36 linker is attached to it for conjugation with nanobody


Source

It can be obtained from the pNeae2 plasmid(https://www.addgene.org/168300/)

References